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He said he did flood his system with the medical marijuana oils for three months. Amy Miller said every day, she is thankful for her husband being healthy and happy again. His dad, his family and strangers helped us come up with cash to go to California and get this treatment. I cried literally the whole way to California.
Darren means everything to me. He is my world and my everything. After this first treatment, the blisters left. Darren Miller is still shocked at the success he has had with his treatment. He said he was at the end of life palliative care stage and simply wanted to die peacefully when everything changed.
I spend three or four hours a night talking to people how I make the oils and try to educate people about it. Darren said he hopes he can be of help to others in the future with whatever questions they might have of what he did. He sees what he has done as something that could be of real help for many people. Now, Darren and Amy Miller, take life a day at a time. He said he tries his best to educate anyone he can about his transformation and how he did it. I want my mission to be to help people who are struggling with this and give them hope.
Subsequent to this paper, evidence has emerged that CBD may contribute anti-anxiety effects to cannabis varieties with THC. See Morgan, Celia J A, et al. See Russo, Ethan B. However, an ideal ratio for CBD: THC may be challenging to achieve because of the complex interactions of both drugs with various receptors.
When the ration is 1. I, Pharmacological interaction between 9-tetrahydrocannabinol and cannabidiol, two active constituents of Cannabis sativa. Influence of administration interval and dose ratio between the cannabinoids. THC is produced primarily by narrow and broad-leafleted drug cannabis varieties. CBD is produced primarily by narrow and broad leafleted fiber cannabis varieties, commonly known as hemp.
Where individual plants are harvested and consumed, breeding for drug cannabis favors selection for THC content. Where fields of drug landrace cannabis are cultivated solely for extraction, cultivars seem to maintain a significantly higher percentage of cannabinoids other than THC, including CBD.
See Clarke, R C et al. Interbreeding drug and hemp varieties of cannabis can produce cultivars that produce both THC and CBD, in amounts that far exceed landrace cannabis drug or fiber varieties. Importantly to the use of medical cannabis , the terpenoid constituents responsible for the appealing aroma and flavor constituents found in the marijuana i. These aroma and flavor terpenoids are also synergistic with THC and CBD, modulating and enhancing the effects of cannabinoids.
Myrcene content in drug cannabis can increase sedation. Limonene can reduce anxiety. Pinene is stimulating and enhances memory. But to-date, these terpenoid attributes have not been available in high-CBD varieties. The present invention provides medical cannabis plants. The strain is known for its abundance of white trichomes and high potency. In some embodiments, the present invention also provides medical cannabis plant varieties with specific ratios of CBD to THC.
The reduction of THC-related adverse effects will result in a better tolerated medicine. In some embodiments, the control cannabis variety is an existing variety, such as a recreational marijuana plant variety.
Variants, mutants and trivial modifications of the seeds, plants, plant parts, plant cells of the present invention can be generated by methods well known and available to one skilled in the art, including but not limited to, mutagenesis e. For more information of mutagenesis in plants, such as agents, protocols, see Acquaah et al. Principles of plant genetics and breeding, Wiley-Blackwell, , ISBN , , which is herein incorporated by reference in its entity. The present invention also relates to a mutagenized population of the cannabis plants of the present invention, and methods of using such populations.
The mutagenized population of the present invention can be used in Targeting Induced Local Lesions in Genomes TILLING screening method, which combines a standard and efficient technique of mutagenesis with a chemical mutagen e. Patent Application Publication No. The present invention also provides any compositions or any products made from or isolated from the plants of the present invention. The present invention provides methods of using the cannabis plants or any parts, any compositions, or any chemicals derived from said plants of the present invention.
In some embodiments, the plants can be used for medical purpose. In some embodiments, the plants can be used by patients having a disease. It exhibits antispasmodic and muscle-relaxant properties as well as stimulates appetite.
Other studies state that cannabis or cannabinoids may be useful in treating alcohol abuse, amyotrophic lateral sclerosis, collagen-induced arthritis, asthma, atherosclerosis, bipolar disorder, colorectal cancer, HIV-Associated Sensory Neuropathy, depression, dystonia, epilepsy, digestive diseases, gliomas, hepatitis C, Huntington's disease, leukemia, skin tumors, methicillin-resistant Staphylococcus aureus MRSA , Parkinson's disease, pruritus, posttraumatic stress disorder PTSD , psoriasis, sickle-cell disease, sleep apnea, and anorexia nervosa.
In some embodiments, the plants of the present invention provide one or more medical benefits to a person in need without any side effects, or with reduced side effects compared to a traditional recreational marijuana plant variety. In some embodiments, the plants can be used for non-medical purposes. In some embodiments, the plants are used for producing food, oil, wax, resin, rope, cloth, pulp, fiber, nutrition, construction material, plastic and composite materials, paper, jewelry, water and soil purification materials, weed control materials, cultivation materials, textiles, clothing, biodegradable plastics, body products, health food and biofuel.
In some embodiments, the plants of the present invention can be used to produce new plant varieties. In some embodiments, the plants are used to develop new, unique and superior varieties or hybrids with desired phenotypes. In some embodiments, selection methods, e. Additional breeding methods have been known to one of ordinary skill in the art, e. Cannabis genome has been sequenced recently Bakel et al. Molecular makers for cannabis plants are described in Datwyler et al.
Genetic variation in hemp and marijuana Cannabis sativa L. Isolation of microsatellite markers in Cannabis sativa L. In some embodiments, said method comprises i crossing any one of the plants of the present invention comprising the expression cassette as a donor to a recipient plant line to create a F1 population; ii selecting offspring that have expression cassette.
Optionally, the offspring can be further selected by testing the expression of the gene of interest. In some embodiments, complete chromosomes of the donor plant are transferred.
For example, the transgenic plant with the expression cassette can serve as a male or female parent in a cross pollination to produce offspring plants, wherein by receiving the transgene from the donor plant, the offspring plants have the expression cassette. In a method for producing plants having the expression cassette, protoplast fusion can also be used for the transfer of the transgene from a donor plant to a recipient plant. Protoplast fusion is an induced or spontaneous union, such as a somatic hybridization, between two or more protoplasts cells in which the cell walls are removed by enzymatic treatment to produce a single bi- or multi-nucleate cell.
The fused cell that may even be obtained with plant species that cannot be interbred in nature is tissue cultured into a hybrid plant exhibiting the desirable combination of traits.
More specifically, a first protoplast can be obtained from a plant having the expression cassette. A second protoplast can be obtained from a second plant line, optionally from another plant species or variety, preferably from the same plant species or variety, that comprises commercially desirable characteristics, such as, but not limited to disease resistance, insect resistance, valuable grain characteristics e. The protoplasts are then fused using traditional protoplast fusion procedures, which are known in the art to produce the cross.
Alternatively, embryo rescue may be employed in the transfer of the expression cassette from a donor plant to a recipient plant. Embryo rescue can be used as a procedure to isolate embryo's from crosses wherein plants fail to produce viable seed. In this process, the fertilized ovary or immature seed of a plant is tissue cultured to create new plants see Pierik, , In vitro culture of higher plants , Springer, ISBN x, , which is incorporated herein by reference in its entirety.
In some embodiments, the recipient plant is an elite line having one or more certain desired traits. Examples of desired traits include but are not limited to those that result in increased biomass production, production of specific chemicals, increased seed production, improved plant material quality, increased seed oil content, etc. Additional examples of desired traits includes pest resistance, vigor, development time time to harvest , enhanced nutrient content, novel growth patterns, flavors or colors, salt, heat, drought and cold tolerance, and the like.
Desired traits also include selectable marker genes e. The recipient plant can also be a plant with preferred chemical compositions, e. In some embodiments, molecular markers are designed and made, based on the genome of the plants of the present application.
Methods of developing molecular markers and their applications are described by Avise Molecular markers, natural history, and evolution, Publisher: Plant biotechnology and molecular markers, Publisher: Science Publishers, , each of which is incorporated by reference in its entirety for all purposes. The molecular markers can be used in molecular marker assisted breeding.
For example, the molecular markers can be utilized to monitor the transfer of the genetic material. In some embodiments, the transferred genetic material is a gene of interest, such as genes that contribute to one or more favorable agronomic phenotypes when expressed in a plant cell, a plant part, or a plant.
Details of existing cannabis plants varieties and breeding methods are described in Potter et al. Evolution and Ethnobotany In press , King, J Cannabible Vols , , and four volumes of Rosenthal's Big Book of Buds series , , , and , each of which is herein incorporated by reference in its entirety for all purposes. Plants of the present invention can be further modified by introducing into the plants one or more transgenes which when expressed lead to desired phenotypes. The most common method for the introduction of new genetic material into a plant genome involves the use of living cells of the bacterial pathogen Agrobacterium tumefaciens to literally inject a piece of DNA, called transfer or T-DNA, into individual plant cells usually following wounding of the tissue where it is targeted to the plant nucleus for chromosomal integration.
There are numerous patents governing Agrobacterium mediated transformation and particular DNA delivery plasmids designed specifically for use with Agrobacterium —for example, U. Agrobacterium -mediated plant transformation involves as a first step the placement of DNA fragments cloned on plasmids into living Agrobacterium cells, which are then subsequently used for transformation into individual plant cells. Agrobacterium -mediated plant transformation is thus an indirect plant transformation method.
Methods of Agrobacterium -mediated plant transformation that involve using vectors with no T-DNA are also well known to those skilled in the art and can have applicability in the present invention. See, for example, U. Direct plant transformation methods using DNA have also been reported. The first of these to be reported historically is electroporation, which utilizes an electrical current applied to a solution containing plant cells M.
A third direct method uses fibrous forms of metal or ceramic consisting of sharp, porous or hollow needle-like projections that literally impale the cells, and also the nuclear envelope of cells. Both silicon carbide and aluminum borate whiskers have been used for plant transformation Mizuno et al. There are other methods reported, and undoubtedly, additional methods will be developed. However, the efficiencies of each of these indirect or direct methods in introducing foreign DNA into plant cells are invariably extremely low, making it necessary to use some method for selection of only those cells that have been transformed, and further, allowing growth and regeneration into plants of only those cells that have been transformed.
For efficient plant transformation, a selection method must be employed such that whole plants are regenerated from a single transformed cell and every cell of the transformed plant carries the DNA of interest. These methods can employ positive selection, whereby a foreign gene is supplied to a plant cell that allows it to utilize a substrate present in the medium that it otherwise could not use, such as mannose or xylose for example, refer U.
More typically, however, negative selection is used because it is more efficient, utilizing selective agents such as herbicides or antibiotics that either kill or inhibit the growth of nontransformed plant cells and reducing the possibility of chimeras. Resistance genes that are effective against negative selective agents are provided on the introduced foreign DNA used for the plant transformation.
However, many different antibiotics and antibiotic resistance genes can be used for transformation purposes refer U. In addition, several herbicides and herbicide resistance genes have been used for transformation purposes, including the bar gene, which confers resistance to the herbicide phosphinothricin White et al.
In addition, the dhfr gene, which confers resistance to the anticancer agent methotrexate, has been used for selection Bourouis et al. Genes can be introduced in a site directed fashion using homologous recombination. Homologous recombination and site-directed integration in plants are discussed in, for example, U. Methods of producing transgenic plants are well known to those of ordinary skill in the art. Transgenic plants can now be produced by a variety of different transformation methods including, but not limited to, electroporation; microinjection; microprojectile bombardment, also known as particle acceleration or biolistic bombardment; viral-mediated transformation; and Agrobacterium -mediated transformation.
The gene gun is used to shoot pellets that are coated with genes e. The gene gun uses an actual explosive. Compressed air or steam may also be used as the propellant. It and its registered trademark are now owned by E. Most species of plants have been transformed using this method. Agrobacterium tumefaciens is a naturally occurring bacterium that is capable of inserting its DNA genetic information into plants, resulting in a type of injury to the plant known as crown gall.
Most species of plants can now be transformed using this method, including cucurbitaceous species. A transgenic plant formed using Agrobacterium transformation methods typically contains a single gene on one chromosome, although multiple copies are possible.
Such transgenic plants can be referred to as being hemizygous for the added gene. A more accurate name for such a plant is an independent segregant, because each transformed plant represents a unique T-DNA integration event U. A heterozygous genotype in which one allele corresponds to the absence of the transgene is also designated hemizygous U. General transformation methods, and specific methods for transforming certain plant species e.
Non-limiting examples of methods for transforming cannabis plants and cannabis tissue culture methods are described in Zweger The Biotechnology of Cannabis sativa, April ; MacKinnon Genetic transformation of Cannabis sativa Linn: Progress towards transformation of fibre hemp, Scottish Crop Research, , and US , each of which is herein incorporated by reference in its entirety for all purposes.
Classical breeding methods can be included in the present invention to introduce one or more recombinant expression cassettes of the present invention into other plant varieties, or other close-related species that are compatible to be crossed with the transgenic plant of the present invention. The improvement of open-pollinated populations of such crops as rye, many maizes and sugar beets, herbage grasses, legumes such as alfalfa and clover, and tropical tree crops such as cacao, coconuts, oil palm and some rubber, depends essentially upon changing gene-frequencies towards fixation of favorable alleles while maintaining a high but far from maximal degree of heterozygosity.
Uniformity in such populations is impossible and trueness-to-type in an open-pollinated variety is a statistical feature of the population as a whole, not a characteristic of individual plants. Thus, the heterogeneity of open-pollinated populations contrasts with the homogeneity or virtually so of inbred lines, clones and hybrids. Population improvement methods fall naturally into two groups, those based on purely phenotypic selection, normally called mass selection, and those based on selection with progeny testing.
Interpopulation improvement utilizes the concept of open breeding populations; allowing genes to flow from one population to another.
Plants in one population cultivar, strain, ecotype, or any germplasm source are crossed either naturally e. Selection is applied to improve one or sometimes both population s by isolating plants with desirable traits from both sources.
There are basically two primary methods of open-pollinated population improvement. First, there is the situation in which a population is changed en masse by a chosen selection procedure. The outcome is an improved population that is indefinitely propagable by random-mating within itself in isolation.
Second, the synthetic variety attains the same end result as population improvement but is not itself propagable as such; it has to be reconstructed from parental lines or clones.
These plant breeding procedures for improving open-pollinated populations are well known to those skilled in the art and comprehensive reviews of breeding procedures routinely used for improving cross-pollinated plants are provided in numerous texts and articles, including: In mass selection, desirable individual plants are chosen, harvested, and the seed composited without progeny testing to produce the following generation. Since selection is based on the maternal parent only, and there is no control over pollination, mass selection amounts to a form of random mating with selection.
As stated herein, the purpose of mass selection is to increase the proportion of superior genotypes in the population. A synthetic variety is produced by crossing inter se a number of genotypes selected for good combining ability in all possible hybrid combinations, with subsequent maintenance of the variety by open pollination.
Whether parents are more or less inbred seed-propagated lines, as in some sugar beet and beans Vicia or clones, as in herbage grasses, clovers and alfalfa, makes no difference in principle. Parents are selected on general combining ability, sometimes by test crosses or toperosses, more generally by polycrosses.
Parental seed lines may be deliberately inbred e. However, even if the parents are not deliberately inbred, selection within lines during line maintenance will ensure that some inbreeding occurs. Clonal parents will, of course, remain unchanged and highly heterozygous. Whether a synthetic can go straight from the parental seed production plot to the farmer or must first undergo one or two cycles of multiplication depends on seed production and the scale of demand for seed.
In practice, grasses and clovers are generally multiplied once or twice and are thus considerably removed from the original synthetic. While mass selection is sometimes used, progeny testing is generally preferred for polycrosses, because of their operational simplicity and obvious relevance to the objective, namely exploitation of general combining ability in a synthetic.
The numbers of parental lines or clones that enter a synthetic vary widely. In practice, numbers of parental lines range from 10 to several hundred, with being the average. Broad based synthetics formed from or more clones would be expected to be more stable during seed multiplication than narrow based synthetics.
A pedigreed variety is a superior genotype developed from selection of individual plants out of a segregating population followed by propagation and seed increase of self pollinated offspring and careful testing of the genotype over several generations. This is an open pollinated method that works well with naturally self pollinating species.
This method can be used in combination with mass selection in variety development. Variations in pedigree and mass selection in combination are the most common methods for generating varieties in self pollinated crops. A hybrid is an individual plant resulting from a cross between parents of differing genotypes.
Commercial hybrids are now used extensively in many crops, including corn maize , sorghum, sugarbeet, sunflower and broccoli.
Hybrids can be formed in a number of different ways, including by crossing two parents directly single cross hybrids , by crossing a single cross hybrid with another parent three-way or triple cross hybrids , or by crossing two different hybrids four-way or double cross hybrids.
Strictly speaking, most individuals in an out breeding i. Heterosis, or hybrid vigor, is usually associated with increased heterozygosity that results in increased vigor of growth, survival, and fertility of hybrids as compared with the parental lines that were used to form the hybrid.
Maximum heterosis is usually achieved by crossing two genetically different, highly inbred lines. This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references, patents and published patent applications cited throughout this application, as well as the Figures are incorporated herein by reference.
One objective of the present invention was to survey landraces of cannabis to identify and establish classes of known cannabis plants, wherein the individual plants of the present invention i. As explained in greater detail below, individual cannabis plants were identified, tested and selected within each landrace to form varieties within each cannabis class. According to the present invention, more than one variety of cannabis may have been established within a single cannabis class.
As explained further herein, selected candidate cannabis plants for a specific variety may have been subjected to further breeding and selection before being chosen as a cannabis variety for a particular class. The final selected varieties were designated as Classes of Cannabis Varieties.
The following Table 2 provides the variety names for each of the Classes of Cannabis Varieties provided by the present invention. The following Table 3 provides the predominant flavors of each Class of Classes of Cannabis Varieties according to the present invention. More detailed descriptions of the development and characteristics of the Classes of Cannabis Varieties of the present invention are provided below, including for the following plant traits:.
Sugar Leaf—leaves surrounding buds inflorescences and that are covered with glandular trichomes. Top of Top—apical most inflorescence body very tip top of plant. Low of Top—lowest auxiliary bud that is part of apical most inflorescence bottom bud of tip top of plant.
Low Mix—ground up lower buds under top canopy. Standard Mix—ground-up combination of lowest, middle and top buds in order to determine an overall average for plant chemotype. As shown further herein, the Classes of Cannabis Varieties of the present invention are used for medical purposes as is or are used in breeding schemes to develop new cannabis varieties within, between or among the various classes of Classes of Cannabis Varieties.
Inflorescences were obtained for a land race of Gold class varieties and seeds from these inflorescences were isolated and put into conditions proper for their germination.
The seeds which germinated grew identically. Of these, the individual phenotype with the best user experience based on testing was selected to create GO13, a variety classified into the Gold Class.
Cuttings from GO13 are marked by 3-finger leaflet sets with internode buds asymmetrically located on alternate sides on main shoot. In particular, the internode space of this variety tends to be greater than that of other gold class varieties and stems harden quicker.
Roots nodes appear with days and roots within days. The GO13 grows extremely tall and thin with extreme stretching and asymmetrical bud and leaf sets. When root system is not limited or pruned, this variety of gold class varieties exhibits unparalleled vigor and stretch.
Vegetative growth is marked by a deep blue-green Munsell ID hue with lime green thin stalks. Petioles are marked by purple pointillism increasing on sides exposed to light and the end closest to palm of the leaflet set. Root bodies are typically full and bright white.
Stalks radiate a pungent smell of body odor or urine. Canopy extremely sparse and apical dominance can be disrupted easily with removal of apical meristem. Main stems also exhibit purpling, but inflorescences are not purple. Leaves are 3 and 5 leaflet patterns with 3 being predominant and overall decreasing to 1 and to none in the presence of female flowers.
Female flowers are spread out due to the large internode spacing. Upon flower set, buds and supporting structures stems, leaves, etc. Again, this variety tends to be more densely covered with trichome bodies than its parent and other gold class varieties.
In fact, the inflorescences are very dense and have large calyxes covered in highly resinous glands that exhibit this variety's distinct lemon Pine-Sol scent after only days. In particular, the oily character of these flowers set this gold class apart from its parent and other gold class varieties. Textures are extremely sticky and fibrous. GO13 consistently produces among the highest THCA levels of medical cannabis known in California and is often noted for an intense and crushing physical effect combined with a sublime and inspiring mental flight.
Noted for excellent appetite and sexual stimulation often accompanied by uninterrupted sleep. Description of Planting, Harvesting and Processing of the Plants. This variety is asexually propagated via taking cuttings of shoots and putting them in rock wool cubes. Full trays were covered, left under 18 hours of light and allowed to root days.
Upon root onset, the plantlets were transplanted into rigid 1 gallon containers filled with a proprietary soil mix A and remain in 18 hours of daylight for another days. Once root bound, plants are transplanted into rigid 3 gallon containers filled with proprietary soil mix B.
They undergo a propriety nutrient regimen and grow as undisturbed as possible for days depending on chemotype analysis. Thus, great care is taken not to disturb the trichome heads and as much of the plant remains intact as possible to promote even and slow drying. In this case, indoor Controlled Environment Agriculture CEA technique following the protocol described elsewhere herein. Potential uses of GO13 include but are not limited to medical applications, as a source for extractions of plant constituents and chemicals, for commercial raw materials, fiber and pulp.
Additionally reduced THCA varieties can be developed that are intended to reduce side-effects from extant recreational cannabis varieties related to GO Flavor when smoked included distinct citrus and mentholated notes. Some patients have compared its flavor to bergamot orange.
Its aroma has been characterized as a tangy, sharp, naphthalene aroma with orange notes and a sweet undertone. While it stimulates appetite, it does not appear to encourage overeating. Low MW Terpene Analysis Mass spectrometry data, chromatography data, and statistical data related to the chemical analysis are shown in the following Tables Most of the acid cannabinoids e. Inflorescences were obtained for a landrace of Haze and seeds from these inflorescences were isolated and put into conditions proper for their germination.
There was absolutely no sweetness in the smell of BRO5. Testing proved that its effects were the most enjoyable and virtually mycrene free. The lack of mycrene and presence of pinene and limonene is quite rare and sets this variety apart from most cannabis varieties. Cuttings from BRO5 are marked by 9-finger very thin leaflet sets with internode buds asymmetrically located on alternate sides on main shoot. In particular, the internode space of this variety tends to be extremely large. Stems are tall, frail and stretchy.
Cuttings roots appear within days. The BRO5 grows tall and stretchy with flimsy stems. It possesses the classic narrow-leafleted morphology associated with 's Haze cultivars that were inherited from Haze's tropical drug cannabis parents, including Colombian and That varieties. BRO5 grows with asymmetrical bud and leaf sets.
Vegetative growth is marked by a lightened green Munsell ID hue with lime green thin stalks. Leaflets are longer and narrower than most of drug cannabis varieties.
There is little or no purple on this plant until the final weeks of flowering. Leaves turn deep purple with flowers silvering up as time goes on. Canopy extremely sparse and topping near flowering is encouraged for even growth. Leaves are 9 and 7 leaflet patterns with 7 being predominant and overall decreasing to 1 and to none in the presence of female flowers.
In particular, flower onset is very slow with this variety. Female flowers are spread apart due to the large internode spacing. Everything about this plant takes longer. As inflorescences mature, they become more hardened and dense.
In particular, the oily character of these flowers was the driving force for selection. BRO5 defines heady, hazy medicine with highly functional mental effects. This variety has the structure and scent of the BRO5 lines famous around the world.
With aromas of spice and anise, the hashish flavor when smoked is enlightening. BRO5 is noted for mood elevation, inspiration and creativity and is also likely to improve home hygiene. Slow drying followed by a one to two month curing process. In this case, indoor CEA technique following the protocol described elsewhere herein. Organic mix of soil in fabric pots, a regimen of nutrients following standard NPK feeding schedules and addition of proprietary mixture.
Potential uses of BRO5 include but are not limited to medical applications, extractions, commercial raw material chemical , fiber and pulp. Patients rave about the great experience of using BRO5.
Inflorescences were obtained for a proprietary breeding program and seeds from these inflorescences were isolated and put into conditions proper for their germination. The resulting plants were then crossed with GO13 plants and seeds were planted and germinated for selection based on oil content of the plants. Plants with higher oil content were selected to create WHI2. Cuttings from WHI2 are marked by 5-finger leaflet sets with internode buds asymmetrically located on alternate sides on main shoot.
Plants are tall, stretchy and productive. Roots of the cuttings appear within days. The WHI2 grows tall and stretchy and exhibits little or no apical dominance. WHI2 grows with asymmetrical bud and leaf sets. Vegetative growth is marked by a lavish green Munsell ID hue with green undersides and hard wood like stalks.
When healthy, fan leaves are extremely jagged and serrations are very pronounced. The stems are strong and fibrous, but extremely thin. The standout quality of WHI2 is the amount of trichomes and their density. Canopy is extremely sparse with clustered bud formation.
Leaves are 5 leaflet patterns with 5 being predominant and overall decreasing to 1 and to none in the presence of female flowers. In particular, flower onset is fast by comparison to most varieties. Trichome density and smell are almost immediate.
Female flowers are spear-shaped, dense and thick although relatively large internode lengths. Again, this variety tends to be more densely covered with trichome bodies than most other varieties. The flowers are compact and well-formed in the shape of small pinecones. As inflorescences mature, the density compact sets compound to form bright orange and silver flowers that give way to yellow and purple sun leaves.
Plants are marked by unusually high oil mass content and extremely dense small resinous buds. Apical inflorescences are often smaller than lowers. Inflorescences particularly are resistant to fungal infestation due to compact oil flowers. The chemotype of this variety is indicative of this diverse genetic heritage. The aroma consists of vanilla, grapefruit, and even has petroleum notes, but a rich creamy vanilla flavor emerges when smoked. Noted for its rare combination of clarity and profound potency, it delivers functional and long lasting inspiration and positivity.
Potential uses of WHI2 include but are not limited to medical applications, extractions, commercial raw material e. Very interesting from an organoleptic standpoint sweet Amsterdam flavor and a caryophyllene content standpoint. WHI2 produces a happy laughing high, with the classic combusted aroma of 's landrace varieties of the same cannabis class.
Chemical analysis for WHI2 plants is summarized in Tables 7 and 8. Inflorescences were obtained for a landrace of SIL4 and seeds from these inflorescences were isolated and put into conditions proper for their germination. The seeds which germinated grew fairly similarly. However, upon flower onset, the seedlings were selected for trichome density, leaflet width and root vigor to create SIL4.
Cuttings from SIL4 are marked by 7-finger leaflet sets with internode buds asymmetrically located on alternate sides on main shoot. In particular, the internode space of this variety tends to be greater than that of other Silver varieties and stems harden more slowly.
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